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One of the biggest myths, repeated over and over by a host of well meaning but ill-informed commentators on social media, is that anaerobic or anoxic conditions can exist in an aquarium in the substrate if the substrate is deep enough. Per these individuals, conditions in some substrates (generally something called a “deep sand bed”) are anaerobic or anoxic and reduce nitrate to nitrogen gas and reduce sulfates to hydrogen sulfide.
Now I know many will “waffle” on this topic as they do not want to offend all these commentators. I cannot do that. All these commentators are just plain wrong! Sorry, can’t sugar coat it.
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Deep sand beds do NOT do ANY anoxic denitrification nor do they create hydrogen sulfide.
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This is true no matter how you set up the deep sand bed. Small sand particle, large sand particle, kitty litter, laterite, gravel, no plenum, plenum, very slow flow, no flow, slow flow, moderate flow, organic matter, no organic matter, three month, three years, etc. etc.. ALL combinations will NOT produce anoxic denitrifying conditions or any hydrogen sulfide gas. It just CANNOT happen for no less than six very solid scientific reasons.
Year long testing of four 5 inch deep sand beds with two controls showed they did NO anoxic denitrification nor did they create any hydrogen sulfide gas.
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Note that the distinction between anoxic and anaerobic is very blurred, with the two terms being used interchangeably in all the literature. We go into the exact meanings in the article linked below on the science but for now assume anaerobic and anoxic mean the same thing.
This anaerobic myth is most commonly associated with something called a “deep sand bed”, where a bed of sand, especially a bed of sand in a marine aquarium, supposedly becomes anoxic and supposedly does a lot of nitrate reduction to nitrogen gas. There is a whole lot of “science” in marine aquarium keeper websites on how “well” this works.
Indeed there is a whole community of hobbyists whose passion is “deep sand beds” or “DSBs”. These hobbyists trade anecdotal evidence about how well DSBs reduce nitrates. This is all simply misinterpretations of what they are seeing. Invariably the real reason for the reduction in nitrates is “assimilatory denitrification” where plants, bacteria, fungi or fish are incorporating the nitrate into their bodies.
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But it is also common for well meaning but ill-informed commentators on social media to say that normal substrates such as sand and gravel have “gone anoxic” and produced hydrogen sulfide gas. They then tell folks this hydrogen sulfide gas killed their fish. This is simply patently impossible in any substrate in an aquarium. Period.
The Anaerobic Science in More Depth
I go into the science behind the anaerobic substrate myth in a great deal more depth in this article (note I recommend this boring lengthy article only for the nerds among us, like myself):
14.2.4.1. The Science behind the Myth of the Anaerobic Aquarium Substrate
For more on the anaerobic myth click on the following:
7.5. Denitrifying Media
8.9. Anaerobic Reactors
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Aquarium Science Website
The chapters shown below or on the right side in maroon lead to close to 400 articles on all aspects of keeping a freshwater aquarium. These articles have NO links to profit making sites and are thus unbiased in their recommendations, unlike all the for-profit sites you will find with Google. Bookmark and browse!
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Dave says
Amber …… Pea gravel, while not optimum, will still work fine in any undergravel set-up. I would simply pull the sponge filter as it is unnecessary. And fine sand will mix well with any of the commercial soil mixes so go ahead with that also.
Amber says
Hi Dave, Thank you for all the information you’ve collected and shared. It’s been most valuable. I’ve followed all of your suggestions and have had great luck with all my different fresh water puffer tanks. I do however have two questions for my established tanks. I have two 60 gal under gravel filter tanks with pea gravel. One ended up with a size a bit above pea gravel mixed . It was frustrating but it seems not to have been the big problem I was stressing about. My first question is if I’m over doing those two tanks by adding a sponge filter . I put it in just to seed the gravel as I had read in one of your many articles. Is this over kill and if so, when can it be taken out.
My second question is I have another established tank with dirt substrate. I forgot the name. But it’s pretty common brand in the pet store. No under gravel filter. I have a canister filter and uv light. The tank has been nice and clear my fish have had no issues while in it for six months. I moved those fish into a bigger tank of 60 gal. This one being a 30 gal. My second question is can I add a few inches of light colored fine sand to cap it off or will this be a problem. I plan to take all the live plants out and make it just rock and wood scraping with places for my fish to shelter while I cycle a bigger 120 gallon tank over the next few months. It will hold a 3/4” fahaka until the new tank is ready. I don’t want to remove the established substrate but he will need sand. Since all my other tanks are under gravel filters I am unsure what this may mean for the well established cycling the tank has undergone. It did hold 4 Amazon puffers until they got the bigger tank. Any suggestions or thoughts are much appreciated. Thank you for your time I’ll look forward to hearing back from you.
Dave says
In reply to Kye For the “best” set up read this. Think five things: HEAVY biofiltration, heavy aeration, light feeding of a high protein food https, and only rearely lightly clean the filters. See this article for the full run down https://aquariumscience.org/index.php/1-1-12-how-to-make-fish-thrive/
Kye says
Hi. I just saw this page in regards to biohome medias, as i was just thinkin of buying some. I really like the way u destroys sooo many beliefs in regards to denitrification. Im gonna bookmark your website and give it a thorough read through. Honestly this is such an interesting read. On a side note, to put it simply, what is the best setup, for a well stocked/ planted tank, according your studies, which is the best bet for livestocks to live safely; better if they thrive.
Thank you, for the tests and everything. Thank you.